Movie #1
GFP:tubulin time-lapse sequence starting before nuclear envelope breakdown and showing spindle assembly and early translocation to the cortex. Spindle shortening and rotation during meiosis I and II are shown but these are not as clear as in
Movie #2
GFP:tubulin time-lapse sequence starting after nuclear envelope breakdown. After the sequence stops jumping around, the parallel orientation of the metaphase spindle, shortening and rotation can be seen clearly through meiosis I and II.
Movie #3
DIC time-lapse sequence showing nuclear envelope breakdown, ovulation, and circular streaming of membrane vesicles during meiosis. The appearance of pronuclei at the end of the movie indicates exit from meiosis.
Movie #4
GFP:vitellogenin time-lapse sequence showing meiosis-specific circular streaming of lysosomes. In this sequence, lysosomes are streaming in the short axis of the embryo.
Movie #5
GFP:vitellogenin time-lapse sequence showing meiosis-specific circular streaming of lysosomes. In this sequence, lysosomes are streaming in the long axis of the embryo.
Movie #6
GFP:tubulin time-lapse sequence showing movement of cytoplasmic microtubules during streaming in a meiotic embryo……
Movie #7
GFP:tubulin and mCherry:histone time lapse sequence showing a meiotic spindle from pro-metaphase I, through spindle translocation, shortening, rotation, and most of anaphase I.
Movie #8
GFP:tubulin, GFP:PH (plasma membrane), and mCherry:histone time lapse sequence showing the events of anaphase I in an embryo with two unpaired X chromosomes. A single chromosome appears to be lagging through anaphase.
Movie #9
GFP:tubulin, GFP:PH (plasma membrane), and mCherry:histone time lapse sequence showing the events of anaphase I in an embryo with two unpaired X chromosomes. A single chromosome lags and is resolved into the forming polar body.